Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Target K_i: 56 nM for protein kinase A

Cell permeable: yes

Primary TargetPKA

General description

Prepared by a chemical modification of K-252a. A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM). Does not significantly affect the activity of PKC, PKG, or MLCK. Inhibits axon branching in cultured neurons.

A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM) that is prepared by a chemical modification of K-252a. Does not significantly affect the activities of PKC, PKG, and MLCK. Inhibits axon branching in cultured neurons. A 2 mM (50 µg/47 µl) solution of KT 5720 (Cat. No. 420323) in DMSO is also available.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Simpson, C.S., and Morris, B.J. 1995. Neuroscience 68, 97.Cabell, L., and Audesirk, G. 1993. Int. J. Dev. Neurosci.11, 357.Gadbois, D.M., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8626.Kase, H., et al. 1987. Biochem. Biophys. Res. Commun.142, 436.

Packaging

100 µg in Glass bottle

50 µg in Plastic ampoule

Preparation Note

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)